WebMay 25, 2024 · Nano Fluorescent standard particles (Cat. No. NFPPS-52-4K, Spherotech Inc. Lake Forest, IL, USA) were used as a reference standard to calibrate the experiment based on particle size and fluorescence. Web(A) Nano Fluorescent Size Standard Kits (NFPPS-52-4K) to calibrate the MPs. (B and C) Representative flow cytometer image of bubble-induced EMPs (Annexin V +). doi:10.1371/journal.pone.0168881.g002 Bubble-Induced EMPs Promote Endothelial Dysfunction PLOS ONE DOI:10.1371/journal.pone.0168881 January 23, 2024 5 / 15 nm …
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Protocol Standardization Reveals MV Correlation to Healthy …
WebThere is a connector I disconnect which eliminates tubing going to our HTS and other possible sub-micron particle noise generating runs. To get our initial settings we run Spherotech NFPPS-52-4K Nano Particle kit, FITC fluor. It is best to start with a FITC Trigger on these beads before you try a Scatter trigger. WebFACS. Nanofluorescent standard particles (Spherotech, Lake Forest, IL, USA; cat# NFPPS-52-4K) were used to set dimensional gate up to 1mm. Routine immunophenotype evaluation was performed on BD FACSCANTO II. Sorting of pure EV population was performed on BD FACSARIA II. Gene expression Conditioned media from osteoblasts were processed for EV WebFeb 1, 2024 · Millipore’s Guava easyCyte 8HT second generation flow cytometer was used with Spherotech’s SPHERO™ Nano Fluorescent Particle Size Standard Kit Beads (Cat No. NFPPS-52-4K). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.) Download : Download high-res image (623KB) christian madu