Top1cc
Web28. nov 2011 · The camptothecin (CPT) Top1 (topoisomerase I) inhibitors exert their anticancer activity by reversibly trapping Top1-DNA cleavage complexes (Top1cc's) and … Web13. feb 2024 · In agreement with the role of Tdp1 in Top1cc repair , Tdp1 knockdown also caused increased accumulation of Top1ccs in Spartan-proficient MEFs (Figure 2C and D and Supplementary Figure S3B). Interestingly, depletion of Tdp1 in Sprtn knockout cells did not cause further Top1cc accumulation compared to Sprtn knockout alone (Figure 2C and D ...
Top1cc
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Web19. feb 2024 · TOP1cc localizes to cytoplasmic chromatin and TOP1 interacts with cGAS to enhance the binding of cGAS to DNA. Retention of TOP1cc to cytoplasmic chromatin … Web26. okt 2016 · Measurement of TOP1cc: CsCl fractionation and immunoblotting. TOP1 protein–DNA complexes (TOP1cc) were purified using caesium chloride density gradients. Cells (2 × 10 6) were lysed in 1% sarcosyl, 8 M guanidine HCl, 30 mM Tris pH 7.5 and 10 mM EDTA. Cell lysates were incubated at 70°C for 15 min to remove all non-covalently bound …
Web23. nov 2024 · Top1-DNA crosslinks accumulate in the tdp1Δ wss1Δ yeast cells lacking the two key Top1cc repair components, the phosphodiesterase Tdp1 and the DPC protease Wss1 (Stingele et al., 2014).To gain insight into the regulatory mechanisms controlling Top1cc processing, we exploited results of a saturated transposon analysis in yeast … Web1. feb 2024 · Top1cc-induced DNA damage response is a complex signaling network that initiates with proteolysis of Top1, thereby allowing subsequent access of DNA repair …
WebTop1cc ChIP was conducted from non-crosslinked exponentially growing cells of yeast strains as in (A). The mean (N = 4) with SEM is shown. Primer-sets location, see (A). Web(D) Top1cc effects on transcription elongation and alternative splicing of HIF-1α mRNA. Transcription levels at two downstream regions from the promoter pausing site spanning exons 1 and 2, and ...
The briefly attached, covalently bonded TOP1-DNA structure at the 3' end of a cleaved DNA single strand is called a TOP1-DNA cleavage complex, or TOP1cc. The TOP1cc is a specific target of TOP1 inhibitors. One of the first inhibitors shown to target TOP1 is irinotecan. Irinotecan is an analogue of the cytotoxic natural alkaloid camptothecin, obtained from the Chinese tree Camptotheca acuminata. Irinotecan is especially effective through its metabolic product SN-38. Ir…
WebTOP1cc produce transcription arrest and generate DSBs that activate ATM, which in turn removes transcription-blocking TOP1cc by promoting TOP1 degradation and TDP1 action. Based on this model, ATM might serve to promote resumption of RNA synthesis and cell survival follow-ing transcription arrest by TOP1cc. The ATM pathway has been shown outside diameter of 2 sch 40 pvc pipeWebCancer-causing mutations often arise from inappropriate DNA repair, yet acute exposure to DNA damage is widely used to treat cancer. The challenge remains in how to specifically induce excessive DNA damage in cancer cells while minimizing the undesirable effects of genomic instability in noncancerous cells. One approach is the acute exposure to … outside diameter of 1.5 inch pipeWebAbstract. Inhibitors of topoisomerase I (Top1) that result in stalled Top1 cleavage complexes (Top1cc) are commonly employed against cancer. Combination … outside diameter of 2 galvanized pipeWeb1. feb 2024 · Selective trapping of human topoisomerase 1 (Top1) on the DNA (Top1 cleavage complexes; Top1cc) by specific Top1-poisons triggers DNA breaks and cell … outside diameter of 3/0 thhnWebTop1cc induce the phosphorylation of TDP1 at serine 81 by the protein kinases ataxia-telangiectasia-mutated kinase (ATM) and DNA-dependent protein kinase (DNA-PK), which … rain shower with waterfallWeb喜树碱是一种有效的拓扑异构酶I（Topoisomerase I, Topo I）抑制剂，不可逆结合DNA-Topo I复合物，抑制Topo I切割后DNA的重新结合，并且以共价连接DNA的方式固定化酶。. 该 … rain signal booster appWeb28. aug 2024 · ATM phosphorylates TDP1 to stabilize it at DSBs produced by the collision of replication and transcription machineries with TOP1cc; this also serves to recruit XRCC1 to facilitate repair of TOP1cc by BER. Additionally, ATM enhances TOP1 proteolysis from DNA which subsequently activates DNA-PK . rain signal today